What are the three types of LAL tests?

What are the three types of LAL tests?

• A. gel clot, spectrophotometric, fluorescent
• B. turbidimetric, colorimetric, gel clot
• C. gel clot, turbidimetric, colorimetric
• D. gel clot, turbidimetric, fluorometric

Answer: (C)

Endotoxin detection with the Limulus amebocyte lysate relies on a cascade that produces a detectable signal when endotoxin is present. The three main readouts are: gel clot, where a firm clot forms if endotoxin is above a threshold; turbidimetric, which measures the increase in turbidity of the reaction to quantify endotoxin; and colorimetric (chromogenic), where a color change from a chromogenic substrate indicates endotoxin presence and concentration. While you can read colorimetric results with a spectrophotometer and there are fluorometric variants, the standard trio is gel clot, turbidimetric, and colorimetric.

Endotoxin detection with the Limulus amebocyte lysate relies on a cascade that produces a detectable signal when endotoxin is present. The three main readouts are: gel clot, where a firm clot forms if endotoxin is above a threshold; turbidimetric, which measures the increase in turbidity of the reaction to quantify endotoxin; and colorimetric (chromogenic), where a color change from a chromogenic substrate indicates endotoxin presence and concentration. While you can read colorimetric results with a spectrophotometer and there are fluorometric variants, the standard trio is gel clot, turbidimetric, and colorimetric.